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Extract from the Marine Seaweed Padina pavonica Protects Mitochondrial Biomembranes from Damage by Amyloidogenic Peptides.

ABSTRACT: The identification of compounds which protect the double-membrane of mitochondrial organelles from disruption by toxic confomers of amyloid proteins may offer a therapeutic strategy to combat human neurodegenerative diseases. Here, we exploited an extract from the marine brown seaweed Padina pavonica (PPE) as a vital source of natural bioactive compounds to protect mitochondrial membranes against insult by oligomeric aggregates of the amyloidogenic proteins amyloid-β (Aβ), α-synuclein (α-syn) and tau, which are currently considered to be major targets for drug discovery in Alzheimer's disease (AD) and Parkinson's disease (PD). We show that PPE manifested a significant inhibitory effect against swelling of isolated mitochondria exposed to the amyloid oligomers, and attenuated the release of cytochrome c from the mitochondria. Using cardiolipin-enriched synthetic lipid membranes, we also show that dye leakage from fluorophore-loaded vesicles and formation of channel-like pores in planar bilayer membranes are largely prevented by incubating the oligomeric aggregates with PPE. Lastly, we demonstrate that PPE curtails the ability of Aβ42 and α-syn monomers to self-assemble into larger β-aggregate structures, as well as potently disrupts their respective amyloid fibrils. In conclusion, the mito-protective and anti-aggregator biological activities of Padina pavonica extract may be of therapeutic value in neurodegenerative proteinopathies, such as AD and PD.

SUBMITTER: Caruana M

PROVIDER: S-EPMC7962105 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Extract from the Marine Seaweed <i>Padina pavonica</i> Protects Mitochondrial Biomembranes from Damage by Amyloidogenic Peptides.

Caruana Mario M Camilleri Angelique A Farrugia Maria Ylenia MY Ghio Stephanie S Jakubíčková Michaela M Cauchi Ruben J RJ Vassallo Neville N

Molecules (Basel, Switzerland) 20210307 5

The identification of compounds which protect the double-membrane of mitochondrial organelles from disruption by toxic confomers of amyloid proteins may offer a therapeutic strategy to combat human neurodegenerative diseases. Here, we exploited an extract from the marine brown seaweed <i>Padina pavonica</i> (PPE) as a vital source of natural bioactive compounds to protect mitochondrial membranes against insult by oligomeric aggregates of the amyloidogenic proteins amyloid-β (Aβ), α-synuclein (α- ...[more]

PMID: 33799979

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Project description:Several studies have shown the importance of using seaweed liquid extract (True-Algae-Max, TAM) as a fish feed additive, and fish-water conditioner. In addition, TAM has demonstrated significant growth improvement when used as a plant growth biostimulant. This study investigates whether seaweed liquid extract (TAM) can achieve good results in new experimental fields such as chromium remediation, plant germination, and live feed supplementation for marine invertebrate Copepod (Oithona nana). In this study, several doses of TAM were tested, for the first time, for their impact on the remediation of chromium (Cr6+) ions from aqueous solutions and as an aqua feed additive for marine copepods (Oithona nana). In addition, it has been tested as promising for the seed germination of Fenugreek (Trigonella foenum-graecum) and Faba bean (Vicia faba L.). The most important factors influencing the removal (%) of Cr6+, identified using a two-level Plackett-Burman factorial design, were selected for additional optimization utilizing a rotatable central composite design. The maximum adsorption of Cr6+ was 93.65% under ideal operating circumstances, which included an initial Cr6+ concentration of 60 mg L-1, a temperature of 25 °C, a pH of 3, a TAM biomass of 0.05 g, and a contact time of 60 min at agitation conditions. Plackett-Burman design data shows the significance of each factor and how well the model fits the Cr6+ removal. The results of the germination experiment revealed that the highest significant increase in seed germination was achieved using a TAM level of 0.30 mg mL-1 with V. faba (88%) and 0.03 mg mL-1 with T. foenum-graecum (96.6%). Additionally, compared to the control group, TAM at a level of 0.037 mg mL-1 showed high root length enhancement on V. faba (184%) and T. foenum-graecum (188%). The results of the copepod O. nana feeding additive experiment found that the group fed on starch supplemented with TAM at a level of 0.3 mL L-1, compared to the control group that fed starch only, showed the highest increment in population growth (134.74%), fecundity (270.16%), and population composition of males (133.45%), adults (120.37%), and nauplius (203.18%). Moreover, compared to the control group, the copepod that fed starch supplemented with TAM levels achieved the highest Omega-9 content. In conclusion, it is shown that TAM is a feasible, efficient, and sustainable solution for biodegradable adsorbent for the Cr6+ from aqueous solution, enhances plant seed germination and root length, and is a novel feed additive for marine copepod O. nana, especially in marine invertebrate hatcheries.

Dataset Information

Extract from the Marine Seaweed Padina pavonica Protects Mitochondrial Biomembranes from Damage by Amyloidogenic Peptides.

Publications

Extract from the Marine Seaweed <i>Padina pavonica</i> Protects Mitochondrial Biomembranes from Damage by Amyloidogenic Peptides.

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