Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture.

ABSTRACT:

Background

Somatic embryogenesis is a phenomenon carried out in an environment that generates abiotic stress. Thus, regenerants may differ from the source of explants at the morphological, genetic, and epigenetic levels. The DNA changes may be the outcome of induction media ingredients (i.e., copper and silver ions) and their concentrations and time of in vitro cultures.

Results

This study optimised the level of copper and silver ion concentration in culture media parallel with the induction medium longevity step towards obtaining barley regenerants via somatic embryogenesis with a minimum or maximum level of tissue culture-induced differences between the donor plant and its regenerants. The optimisation process is based on tissue culture-induced variation evaluated via the

SUBMITTER: Orlowska R 

PROVIDER: S-EPMC7962293 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature