P25 Gene Knockout Contributes to Human Epidermal Growth Factor Production in Transgenic Silkworms.

ABSTRACT: Transgenic silkworm expression systems have been applied for producing various recombinant proteins. Knocking out or downregulating an endogenous silk protein is considered a viable strategy for improving the ability of transgenic expression systems to produce exogenous proteins. Here, we report the expression of human epidermal growth factor (hEGF) in a P25 gene knockout silkworm. The hEGF gene regulated by the P25 gene promoter was integrated into a silkworm's genome. Five transgenic positive silkworm lineages were generated with different insertion sites on silkworm chromosomes and the ability to synthesize and secrete proteins into cocoons. Then, a cross-strategy was used to produce transgenic silkworms with a P25 gene knockout background. The results of the protein analysis showed tha

SUBMITTER: Wu M 

PROVIDER: S-EPMC7962452 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature