Identification of m⁶A residues at single-nucleotide resolution using eCLIP and an accessible custom analysis pipeline.

ABSTRACT: Methylation at the N⁶ position of adenosine (m⁶A) is one of the most abundant RNA modifications found in eukaryotes; however, accurate detection of specific m⁶A nucleotides within transcripts has been historically challenging due to m⁶A and unmodified adenosine having virtually indistinguishable chemical properties. While previous strategies such as methyl-RNA immunoprecipitation and sequencing (MeRIP-seq) have relied on m⁶A-specific antibodies to isolate RNA fragments containing the modification, these methods do not allow for precise identification of individual m⁶A residues. More recently, modified cross-linking and immunoprecipitation (CLIP)-based approaches that rely on inducing specific mutations during reverse transcr

SUBMITTER: Roberts JT 

PROVIDER: S-EPMC7962486 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature