Fluorogenic aptamers resolve the flexibility of RNA junctions using orientation-dependent FRET.

ABSTRACT: To further understand the transcriptome, new tools capable of measuring folding, interactions, and localization of RNA are needed. Although Förster resonance energy transfer (FRET) is an angle- and distance-dependent phenomenon, the majority of FRET measurements have been used to report distances, by assuming rotationally averaged donor-acceptor pairs. Angle-dependent FRET measurements have proven challenging for nucleic acids due to the difficulties in incorporating fluorophores rigidly into local substructures in a biocompatible manner. Fluorescence turn-on RNA aptamers are genetically encodable tags that appear to rigidly confine their cognate fluorophores, and thus have the potential to report angular-resolved FRET. Here, we use the fluorescent aptamers Broccoli and Mango-III as donor

SUBMITTER: Jeng SCY 

PROVIDER: S-EPMC7962493 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature