Effect of Carbon Ion Radiation Induces Bystander Effect on Metastasis of A549 Cells and Metabonomic Correlation Analysis.

ABSTRACT:

Objective

To analyze the effect of carbon ion (¹²C⁶⁺) radiation may induce bystander effect on A549 cell metastasis and metabonomics.

Methods

A549 cell was irradiated with carbon ion to establish the clone survival model and the transwell matrix assay was applied to measure the effect of carbon ion on cell viability, migration, and invasion, respectively. Normal human embryonic lung fibroblasts (WI-38) were irradiated with carbon ions of 0 and 2 Gy and then transferred to A549 cell co-culture medium for 24 h. The migration and invasion of A549 cells were detected by the Transwell chamber. The analysis of metabonomic information in transfer medium by liquid phase mass spectrometry (LC-MS), The differential molecules were obtained by principal pomponent a

SUBMITTER: Yang Z 

PROVIDER: S-EPMC7962605 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature