A competing endogenous RNA network reveals key lncRNAs associated with sepsis.

ABSTRACT:

Background

This study set out to determine key lncRNAs correlated with sepsis via constructing competing endogenous RNA (ceRNA) network.

Methods

Three septic patients and three healthy controls were recruited to obtain lncRNA profiles in this current study. Combined with the mRNA profiles by RNA-sequencing, an integrated analysis of mRNA expression profiles downloaded from GEO was performed to obtain the differentially expressed mRNAs (DEmRNAs). Based on differentially expressed lncRNAs (DElncRNAs) and DEmRNAs acquired in this present study and differentially expressed miRNAs (DEmiRNAs) acquired in previous study, a ceRNA network was constructed. Furthermore, LINC00963 was validated in THP-1 cells by performing loss of function assays.

Results

In this analysis, a tota

SUBMITTER: Guo X 

PROVIDER: S-EPMC7963432 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature