MetaHiC phage-bacteria infection network reveals active cycling phages of the healthy human gut.

ABSTRACT: Bacteriophages play important roles in regulating the intestinal human microbiota composition, dynamics, and homeostasis, and characterizing their bacterial hosts is needed to understand their impact. We applied a metagenomic Hi-C approach on 10 healthy human gut samples to unveil a large infection network encompassing more than 6000 interactions bridging a metagenomic assembled genomes (MAGs) and a phage sequence, allowing to study in situ phage-host ratio. Whereas three-quarters of these sequences likely correspond to dormant prophages, 5% exhibit a much higher coverage than their associated MAG, representing potentially actively replicating phages. We detected 17 sequences of members of the crAss-like phage family, whose hosts diversity remained until recently relatively elusive. For ea

SUBMITTER: Marbouty M 

PROVIDER: S-EPMC7963479 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature