Estimating transition path times and shapes from single-molecule photon trajectories: A simulation analysis.

ABSTRACT: In a two-state molecular system, transition paths comprise the portions of trajectories during which the system transits from one stable state to the other. Because of their low population, it is essentially impossible to obtain information on transition paths from experiments on a large sample of molecules. However, single-molecule experiments such as laser optical tweezers or Förster resonance energy transfer (FRET) spectroscopy have allowed transition-path durations to be estimated. Here, we use molecular simulations to test the methodology for obtaining information on transition paths in single-molecule FRET by generating photon trajectories from the distance trajectories obtained in the simulation. Encouragingly, we find that this maximum likelihood analysis yields transition-path tim

SUBMITTER: Taumoefolau GH 

PROVIDER: S-EPMC7963489 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature