Cellular responses to deproteinized bovine bone mineral biofunctionalized with bone-conditioned medium.

ABSTRACT:

Objectives

The aim of the study was to investigate whether the osteoinductive properties of bone-conditioned medium (BCM) harvested from cortical bone chips within a clinically relevant short-term period can enhance the biologic characteristics of deproteinized bovine bone mineral (DBBM) in vitro.

Materials and methods

To assess the biofunctionalization of DBBM, the adhesive, proliferative, and differentiation properties of mesenchymal stromal ST2, pre-osteoblastic MC3T3-E1, and primary bone-derived cells grown on BCM-coated DBBM were examined by crystal violet staining of adherent cells, BrdU ELISA, and qRT-PCR, respectively.

Results

BCM extracted within 20 min or 24 h in either Ringer's solution (BCM-RS) or RS mixed with autologous serum (BCM-RS + S) increased the a

SUBMITTER: Parisi L 

PROVIDER: S-EPMC7966141 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature