Identifying CNS-colonizing T cells as potential therapeutic targets to prevent progression of multiple sclerosis.

ABSTRACT:

Background

Multiple sclerosis (MS), an autoimmune disease of the central nervous system (CNS), can be suppressed in its early stages but eventually becomes clinically progressive and unresponsive to therapy. Here, we investigate whether the therapeutic resistance of progressive MS can be attributed to chronic immune cell accumulation behind the blood-brain barrier (BBB).

Methods

We systematically track CNS-homing immune cells in the peripheral blood of 31 MS patients and 31 matched healthy individuals in an integrated analysis of 497,705 single-cell transcriptomes and 355,433 surface protein profiles from 71 samples. Through spatial RNA sequencing, we localize these cells in post mortem brain tissue of 6 progressive MS patients contrasted against 4 control brains (20

SUBMITTER: Kaufmann M 

PROVIDER: S-EPMC7966680 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature