Exploring antibiotic resistance in environmental integron-cassettes through intI-attC amplicons deep sequencing.

ABSTRACT:

Introduction

Freshwater ecosystems provide propitious conditions for the acquisition and spread of antibiotic resistance genes (ARGs), and integrons play an important role in this process.

Material and methods

In the present study, the diversity of putative environmental integron-cassettes, as well as their potential bacterial hosts in the Velhas River (Brazil), was explored through intI-attC and 16S rRNA amplicons deep sequencing. RESULTS AND DISCUSSION: ORFs related to different biological processes were observed, from DNA integration to oxidation-reduction. ARGs-cassettes were mainly associated with class 1 mobile integrons carried by pathogenic Gammaproteobacteria, and possibly sedentary chromosomal integrons hosted by Proteobacteria and Actinobacteria. Two putative nove

SUBMITTER: Dias MF 

PROVIDER: S-EPMC7966681 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature