Dynamic consolidated bioprocessing for innovative lab-scale production of bacterial alkaline phosphatase from Bacillus paralicheniformis strain APSO.

ABSTRACT: To meet the present and forecasted market demand, bacterial alkaline phosphatase (ALP) production must be increased through innovative and efficient production strategies. Using sugarcane molasses and biogenic apatite as low-cost and easily available raw materials, this work demonstrates the scalability of ALP production from a newfound Bacillus paralicheniformis strain APSO isolated from a black liquor sample. Mathematical experimental designs including sequential Plackett-Burman followed by rotatable central composite designs were employed to select and optimize the concentrations of the statistically significant media components, which were determined to be molasses, (NH₄)₂NO₃, and KCl. Batch cultivation in a 7-L stirred-tank bioreactor under uncontrolle

SUBMITTER: Abdelgalil SA 

PROVIDER: S-EPMC7966758 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature