Crosslinking of human plasma C-reactive protein to human serum albumin via disulfide bond oxidation.

ABSTRACT: Inter- and intra-molecular crosslinks can generate protein dysfunction, and are associated with protein aggregate accumulation in aged and diseased tissues. Crosslinks formed between multiple amino acid side chains can be reversible or irreversible. Disulfides formed either enzymatically, or as a result of oxidant-mediated reactions, are a major class of reversible crosslinks. Whilst these are commonly generated via oxidation of Cys thiol groups, they are also formed by 'oxidant-mediated thiol-disulfide reactions' via initial disulfide oxidation to a thiosulfinate or zwitterionic peroxide, and subsequent reaction with another thiol including those on other proteins. This generates new intermolecular protein-protein crosslinks. Here we demonstrate that photooxidation, or reaction with the b

SUBMITTER: Jiang S 

PROVIDER: S-EPMC7966873 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature