Distinct DNA Methylation Patterns of Rheumatoid Arthritis Peripheral Blood and Synovial Tissue T Cells.

ABSTRACT:

Objective

To study epigenetic patterns in T lymphocytes that accumulate in the rheumatoid arthritis (RA) synovium, we characterized DNA methylation of CD3⁺ T cells in peripheral blood and synovial tissue in patients with RA and osteoarthritis (OA).

Methods

Genomic DNA of CD3⁺ T cells was isolated from patients with RA (n = 8) and OA (n = 5) from blood or the synovium at the time of an arthroplasty using antibodies and magnetic beads. Methylation was measured by using the Illumina Infinium MethylationEPIC Kit. Differentially methylated loci (DML) and differentially methylated genes (DMGs) were identified by using Welch's t-test. Principal component analysis, hierarchical clustering, and pathway analysis were used to determine relationships among groups.<

SUBMITTER: Ai R 

PROVIDER: S-EPMC7966880 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature