CryptoGenotyper: A new bioinformatics tool for rapid Cryptosporidium identification.

ABSTRACT: Cryptosporidium is a protozoan parasite that is transmitted to both humans and animals through zoonotic or anthroponotic means. When a host is infected with this parasite, it causes a gastrointestinal disease known as cryptosporidiosis. To understand the transmission dynamics of Cryptosporidium, the small subunit (SSU or 18S) rRNA and gp60 genes are commonly studied through PCR analysis and conventional Sanger sequencing. However, analyzing sequence chromatograms manually is both time consuming and prone to human error, especially in the presence of poorly resolved, heterozygous peaks and the absence of a validated database. For this study, we developed a Cryptosporidium genotyping tool, called CryptoGenotyper, which has the capability to read raw Sanger sequenc

SUBMITTER: Yanta CA 

PROVIDER: S-EPMC7966988 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature