Rational Design of Adenylate Kinase Thermostability through Coevolution and Sequence Divergence Analysis.

ABSTRACT: Protein engineering is actively pursued in industrial and laboratory settings for high thermostability. Among the many protein engineering methods, rational design by bioinformatics provides theoretical guidance without time-consuming experimental screenings. However, most rational design methods either rely on protein tertiary structure information or have limited accuracies. We proposed a primary-sequence-based algorithm for increasing the heat resistance of a protein while maintaining its functions. Using adenylate kinase (ADK) family as a model system, this method identified a series of amino acid sites closely related to thermostability. Single- and double-point mutants constructed based on this method increase the thermal denaturation temperature of the mesophilic Escherichia coli

SUBMITTER: Chang J 

PROVIDER: S-EPMC7967156 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature