Oxygen concentration alters mitochondrial structure and function in in vitro fertilized preimplantation mouse embryos.

ABSTRACT:

Study question

Does the oxygen concentration in the culture medium [either physiologic (5%) or atmospheric (20%)] affect mitochondrial ultrastructure and function in preimplantation mouse embryos generated by IVF?

Summary answer

Embryos cultured in 20% oxygen show increased mitochondrial abnormalities compared to embryos cultured in 5% oxygen.

What is known already

ART are widely used and have resulted in the birth of more than 8 million children. A variety of media and oxygen concentrations are used to culture embryos. Embryos cultured under physiological O2 tension (5%) reach the blastocyst stage faster and have fewer alterations in gene expression when compared with embryos cultured under atmospheric oxygen conditions (20%). The mechanisms by which oxygen tension a

SUBMITTER: Belli M 

PROVIDER: S-EPMC7967792 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature