Immune gene expression in kidney biopsies of lupus nephritis patients at diagnosis and at renal flare.

ABSTRACT:

Background

Up to 50% of lupus nephritis (LN) patients experience renal flares after their initial episode of LN. These flares contribute to poor renal outcomes. We postulated that intrarenal immune gene expression is different in flares compared with de novo LN, and conducted these studies to test this hypothesis.

Methods

Glomerular and tubulointerstitial immune gene expression was evaluated in 14 patients who had a kidney biopsy to diagnose LN and another biopsy at their first LN flare. Ten healthy living kidney donors were included as controls. RNA was extracted from laser microdissected formalin-fixed paraffin-embedded kidney biopsies. Gene expression was analyzed using the Nanostring nCounter® platform and validated by quantitative real-time polymerase chain reaction. Di

SUBMITTER: Mejia-Vilet JM 

PROVIDER: S-EPMC7967887 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature