Development and validation of genome-wide InDel markers with high levels of polymorphism in bitter gourd (Momordica charantia).

ABSTRACT:

Background

The preferred choice for molecular marker development is identifying existing variation in populations through DNA sequencing. With the genome resources currently available for bitter gourd (Momordica charantia), it is now possible to detect genome-wide insertion-deletion (InDel) polymorphisms among bitter gourd populations, which guides the efficient development of InDel markers.

Results

Here, using bioinformatics technology, we detected 389,487 InDels from 61 Chinese bitter gourd accessions with an average density of approximately 1298 InDels/Mb. Then we developed a total of 2502 unique InDel primer pairs with a polymorphism information content (PIC) ≥0.6 distributed across the whole genome. Amplification of InDels in two bitter gourd lines '47-2-1-1-3' and '04-

SUBMITTER: Cui J 

PROVIDER: S-EPMC7968231 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature