Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

ABSTRACT:

Background

Tbx2 encodes a transcriptional repressor implicated in the development of numerous organs in mouse. During lung development TBX2 maintains the proliferation of mesenchymal progenitors, and hence, epithelial proliferation and branching morphogenesis. The pro-proliferative function was traced to direct repression of the cell-cycle inhibitor genes Cdkn1a and Cdkn1b, as well as of genes encoding WNT antagonists, Frzb and Shisa3, to increase pro-proliferative WNT signaling. Despite these important molecular insights, we still lack knowledge of the DNA occupancy of TBX2 in the genome, and of the protein interaction partners involved in transcriptional repression of target genes.

Methods

We used chromatin immunoprecipitation (ChIP)-sequencing and expression analyses to i

SUBMITTER: Ludtke TH 

PROVIDER: S-EPMC7968368 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature