Targeting MMP-14 for dual PET and fluorescence imaging of glioma in preclinical models.

ABSTRACT:

Purpose

There is a clinical need for agents that target glioma cells for non-invasive and intraoperative imaging to guide therapeutic intervention and improve the prognosis of glioma. Matrix metalloproteinase (MMP)-14 is overexpressed in glioma with negligible expression in normal brain, presenting MMP-14 as an attractive biomarker for imaging glioma. In this study, we designed a peptide probe containing a near-infrared fluorescence (NIRF) dye/quencher pair, a positron emission tomography (PET) radionuclide, and a moiety with high affinity to MMP-14. This novel substrate-binding peptide allows dual modality imaging of glioma only after cleavage by MMP-14 to activate the quenched NIRF signal, enhancing probe specificity and imaging contrast.

Methods

MMP-14 expression and acti

SUBMITTER: Kasten BB 

PROVIDER: S-EPMC7968515 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature