1D ¹ H NMR as a Tool for Fecal Metabolomics.

ABSTRACT: Metabolomic studies allow a deeper understanding of the processes of a given ecological community than nucleic acid-based surveys alone. In the case of the gut microbiota, a metabolic profile of, for example, a fecal sample provides details about the function and interactions within the distal region of the gastrointestinal tract, and such a profile can be generated in a number of different ways. This unit elaborates on the use of 1D ¹ H NMR spectroscopy as a commonly used method to characterize small-molecule metabolites of the fecal metabonome (meta-metabolome). We describe a set of protocols for the preparation of fecal water extraction, storage, scanning, measurement of pH, and spectral processing and analysis. We also compare the effects of various sample storage conditions

SUBMITTER: Ganobis CM 

PROVIDER: S-EPMC7968567 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature