Dataset Information

Adaptive immunity to human coronaviruses is widespread but low in magnitude.

ABSTRACT:

Objectives

Endemic human coronaviruses (hCoVs) circulate worldwide but cause minimal mortality. Although seroconversion to hCoV is near ubiquitous during childhood, little is known about hCoV-specific T-cell memory in adults.

Methods

We quantified CD4 T-cell and antibody responses to hCoV spike antigens in 42 SARS-CoV-2-uninfected individuals. Antigen-specific memory T cells and circulating T follicular helper (cTFH) cells were identified using an activation-induced marker assay and characterised for memory phenotype and chemokine receptor expression.

Results

T-cell responses were widespread within conventional memory and cTFH compartments but did not correlate with IgG titres. SARS-CoV-2 cross-reactive T cells were observed in 48% of participants and correlated with HKU1 memory. hCoV-specific T cells exhibited a CCR6⁺ central memory phenotype in the blood, but were enriched for frequency and CXCR3 expression in human lung-draining lymph nodes.

Conclusion

Overall, hCoV-specific humoral and cellular memory are independently maintained, with a shared phenotype existing among coronavirus-specific CD4 T cells. This understanding of endemic coronavirus immunity provides insight into the homeostatic maintenance of immune responses that are likely to be critical components of protection against SARS-CoV-2.

SUBMITTER: Tan HX

PROVIDER: S-EPMC7968850 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

Adaptive immunity to human coronaviruses is widespread but low in magnitude.

Tan Hyon-Xhi HX Lee Wen Shi WS Wragg Kathleen M KM Nelson Christina C Esterbauer Robyn R Kelly Hannah G HG Amarasena Thakshila T Jones Robert R Starkey Graham G Wang Bao Zhong BZ Yoshino Osamu O Tiang Thomas T Grayson Michael Lindsay ML Opdam Helen H D'Costa Rohit R Vago Angela A Mackay Laura K LK Gordon Claire L CL Wheatley Adam K AK Kent Stephen J SJ Juno Jennifer A JA

Clinical & translational immunology 20210317 3

<h4>Objectives</h4>Endemic human coronaviruses (hCoVs) circulate worldwide but cause minimal mortality. Although seroconversion to hCoV is near ubiquitous during childhood, little is known about hCoV-specific T-cell memory in adults.<h4>Methods</h4>We quantified CD4 T-cell and antibody responses to hCoV spike antigens in 42 SARS-CoV-2-uninfected individuals. Antigen-specific memory T cells and circulating T follicular helper (cTFH) cells were identified using an activation-induced marker assay a ...[more]

PMID: 33747512

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Dataset Information

Adaptive immunity to human coronaviruses is widespread but low in magnitude.

Objectives

Methods

Results

Conclusion

Publications

Adaptive immunity to human coronaviruses is widespread but low in magnitude.

Similar Datasets

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