Comparative computer-assisted sperm analysis in non-human primates.

ABSTRACT:

Background

Biomedical research has recently focused on developing new models of human disease by implementing genome-editing strategies in non-human primates (NHPs) to introduce relevant gene mutations. There is a need to establish objective semen evaluation methods to select sires for in vitro fertilization to perform germline editing in embryos.

Methods

Sperm motility kinematic parameters were evaluated using a computer-assisted semen analysis (CASA) instrument for rhesus macaques (Macaca mulatta), cynomolgus macaques (Macaca fascicularis), and common marmosets (Callithrix jacchus).

Results

Normative sperm kinematic parameters were established, revealing differences between marmosets and macaques. The impact of season on rhesus macaque sperm motility was modest, whe

SUBMITTER: Schmidt JK 

PROVIDER: S-EPMC7969417 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature