Gene Expression Profile Is Different between Intact and Enzymatically Digested Equine Articular Cartilage.

ABSTRACT:

Objectives

RNA isolation is necessary for the evaluation of gene expression. Due to the nature of its extracellular matrix, RNA isolation from articular hyaline cartilage is difficult and thus the tissue is commonly enzymatically digested in order to extract RNA from the obtained chondrocytes. We hypothesized that the digestion process affects the expression levels of common cartilage-associated genes.

Design

Expression of cartilage-associated genes was compared between intact cartilage and digested chondrocytes from weight bearing and non-weight bearing regions of the equine fetlock joint.

Results

The gene expression of SOX9, COL1A2, COL2A1, ACAN, and COLX were analyzed. Digested cartilage showed a significant decrease in the expression of COL1A2, CO

SUBMITTER: Lepage SIM 

PROVIDER: S-EPMC7970368 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature