Exosome-Mediated Crosstalk between Keratinocytes and Macrophages in Cutaneous Wound Healing.

ABSTRACT: Bidirectional cell-cell communication involving exosome-borne cargo such as miRNA has emerged as a critical mechanism for wound healing. Unlike other shedding vesicles, exosomes selectively package miRNA by SUMOylation of heterogeneous nuclear ribonucleoproteinA2B1 (hnRNPA2B1). In this work, we elucidate the significance of exosome in keratinocyte-macrophage crosstalk following injury. Keratinocyte-derived exosomes were genetically labeled with GFP-reporter (Exo_κ-GFP) using tissue nanotransfection (TNT), and they were isolated from dorsal murine skin and wound-edge tissue by affinity selection using magnetic beads. Surface N-glycans of Exo_κ-GFP were also characterized. Unlike skin exosome, wound-edge Exo_κ-GFP demonstrated characteristic N-glycan ions with a

SUBMITTER: Zhou X 

PROVIDER: S-EPMC7970718 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature