Identification and recombinant expression of an antimicrobial peptide (cecropin B-like) from soybean pest Anticarsia gemmatalis.

ABSTRACT:

Abstract

Background

Insects can be found in numerous diverse environments, being exposed to pathogenic organisms like fungi and bacteria. Once these pathogens cross insect physical barriers, the innate immune system operates through cellular and humoral responses. Antimicrobial peptides are small molecules produced by immune signaling cascades that develop an important and generalist role in insect defenses against a variety of microorganisms. In the present work, a cecropin B-like peptide (AgCecropB) sequence was identified in the velvetbean caterpillar Anticarsia gemmatalis and cloned in a bacterial plasmid vector for further heterologous expression and antimicrobial tests.

Methods

AgCecropB sequence (without the signal peptide) was cloned in the plasmid vec

SUBMITTER: Ramos LFC 

PROVIDER: S-EPMC7970720 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature