Leader RNA regulates snakehead vesiculovirus replication via interacting with viral nucleoprotein.

ABSTRACT: Leader RNA, a kind of virus-derived small noncoding RNA, has been proposed to play an important role in regulating virus replication, but the underlying mechanism remains elusive. In this study, snakehead vesiculovirus (SHVV), a kind of fish rhabdovirus causing high mortality to the cultured snakehead fish in China, was used to unveil the molecular function of leader RNA. High-throughput small RNA sequencing of SHVV-infected cells showed that SHVV produced two groups of leader RNAs (named le_group1 and le_group2) during infection. Overexpression and knockout experiments reveal that le_group1, but not le_group2, affects SHVV replication. Mechanistically, le_group1-mediated regulation of SHVV replication was associated with its interaction wi

SUBMITTER: Qin X 

PROVIDER: S-EPMC7971199 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature