Microenvironmental determinants of organized iPSC-cardiomyocyte tissues on synthetic fibrous matrices.

ABSTRACT: Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) show great potential for engineering myocardium to study cardiac disease and create regenerative therapies. However, iPSC-CMs typically possess a late embryonic stage phenotype, with cells failing to exhibit markers of mature adult tissue. This is due in part to insufficient knowledge and control of microenvironmental cues required to facilitate the organization and maturation of iPSC-CMs. Here, we employed a cell-adhesive, mechanically tunable synthetic fibrous extracellular matrix (ECM) consisting of electrospun dextran vinyl sulfone (DVS) fibers and examined how biochemical, architectural, and mechanical properties of the ECM impact iPSC-CM tissue assembly and subsequent function. Exploring a multidimensional paramete

SUBMITTER: DePalma SJ 

PROVIDER: S-EPMC7971708 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature