A multiscale model via single-cell transcriptomics reveals robust patterning mechanisms during early mammalian embryo development.

ABSTRACT: During early mammalian embryo development, a small number of cells make robust fate decisions at particular spatial locations in a tight time window to form inner cell mass (ICM), and later epiblast (Epi) and primitive endoderm (PE). While recent single-cell transcriptomics data allows scrutinization of heterogeneity of individual cells, consistent spatial and temporal mechanisms the early embryo utilize to robustly form the Epi/PE layers from ICM remain elusive. Here we build a multiscale three-dimensional model for mammalian embryo to recapitulate the observed patterning process from zygote to late blastocyst. By integrating the spatiotemporal information reconstructed from multiple single-cell transcriptomic datasets, the data-informed modeling analysis suggests two major processes crit

SUBMITTER: Cang Z 

PROVIDER: S-EPMC7971879 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature