Optimizations for identifying reference genes in bone and cartilage bioengineering.

ABSTRACT:

Background

Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) remains one of the best-established techniques to assess gene expression patterns. However, appropriate reference gene(s) selection remains a critical and challenging subject in which inappropriate reference gene selction can distort results leading to false interpretations. To date, mixed opinions still exist in how to choose the most optimal reference gene sets in accodrance to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guideline. Therefore, the purpose of this study was to investigate which schemes were the most feasible for the identification of reference genes in a bone and cartilage bioengineering experimental setting. In this study, rat bo

SUBMITTER: Xiong F 

PROVIDER: S-EPMC7972220 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature