Fabrication of methotrexate-loaded gold nanoconjugates and its enhanced anticancer activity in breast cancer.

ABSTRACT: Methotrexate (MTX) is known antagonist of folic acid and widely used as an anti-cancer drug. The folate receptor (FR) and reduced folate carrier are mostly responsible for internalization of methotrexate in tumor cells. Mutation in reduced folate carrier (RFC) leads to resistance against MTX in various tumor cell lines including MDA-MB-231 breast cancer cells. To overcome the resistance of MTX, folate receptor targeted nanoparticles have been commonly used for targeting breast tumors. The aim of the study is to determine the ability of methotrexate gold nanoparticles (MTX-GNPs) in the induction of apoptosis and to explore the molecular changes at genomics and proteomics level. Different assays like cell viability assay, cell cycle analysis, apoptosis, real-time PCR and western blot were ca

SUBMITTER: Chaudhari R 

PROVIDER: S-EPMC7973353 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature