Bioprospecting of the agaricomycete Ganoderma australe GPC191 as novel source for L-asparaginase production.

ABSTRACT: L-Asparaginase is a therapeutically and industrially-competent enzyme, acting predominantly as an anti-neoplastic and anti-cancerous agent. The existing formulations of prokaryotic L-asparaginase are often toxic and contain L-glutaminase and urease residues, thereby increasing the purification steps. Production of L-glutaminase and urease free L-asparaginase is thus desired. In this research, bioprospecting of isolates from the less explored class Agaricomycetes was undertaken for L-asparaginase production. Plate assay (using phenol red and bromothymol blue dyes) was performed followed by estimation of L-asparaginase, L-glutaminase and urease activities by Nesslerization reaction for all the isolates. The isolate displaying the desired enzyme production was subjected to morphological, mole

SUBMITTER: Chakraborty M 

PROVIDER: S-EPMC7973716 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature