The effects of the miR-21/SMAD7/TGF-β pathway on Th17 cell differentiation in COPD.

ABSTRACT: Chronic obstructive pulmonary disease (COPD) is a complex disease with multiple etiologies, while smoking is the most established one. The present study investigated the modulation of T-helper 17 (Th17) cell differentiation by the miR-21/Smad7/TGF-β pathway, and their roles in COPD. Lung tissues were obtained from lung cancer patients with or without COPD who underwent lobotomy and the levels of miR-21, TGF-β/Smad signaling molecules, RORγT, and other Th17-related cytokines were detected. Mouse COPD models were built by exposing both wild-type (WT) and miR-21^-/- mice to cigarette smoke (CS) and cigarette smoke extract (CSE) intraperitoneal injection. Isolated primary CD4⁺ T cells were treated with either CS extract, miR-21 mimics or inhibitors, followed by measuring T

SUBMITTER: He S 

PROVIDER: S-EPMC7973755 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature