MiR‑320a‑3P alleviates the epithelial‑mesenchymal transition of A549 cells by activation of STAT3/SMAD3 signaling in a pulmonary fibrosis model.

ABSTRACT: Pulmonary fibrosis (PF) is a common, chronic and incurable lung disease, in which the lungs become scarred over time. MicroRNAs (miRNAs/miRs) serve key roles in various biological processes, including cell proliferation, differentiation, apoptosis and the regulation of epithelial‑mesenchymal transition (EMT) process. The aim of the present study was to investigate the underlying mechanism of miR‑320a‑3p as a potential therapeutic target for PF. Clinical samples and microarray datasets collected from various databases were used to evaluate the expression of miR‑320a‑3p in PF. A549 cells were used to construct an EMT model of PF. A dual‑luciferase reporter assay system was used to identify target genes of miR‑320a‑3p. Western blot analysis and immunofluorescence staining were used to determi

SUBMITTER: Wang X 

PROVIDER: S-EPMC7974326 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature