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Complete Genome Sequences of Three Uropathogenic Klebsiella quasipneumoniae Strains Isolated from Postmenopausal Women with Recurrent Urinary Tract Infection.

ABSTRACT: Recurrent urinary tract infection (rUTI) poses a major health issue, especially among postmenopausal women. We report complete genome sequences of three Klebsiella quasipneumoniae strains isolated from the urine of postmenopausal women with rUTI. K. quasipneumoniae is a recently identified Klebsiella species with clinical and virulence characteristics distinct from those of K. pneumoniae.

SUBMITTER: Venkitapathi S

PROVIDER: S-EPMC7975875 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Complete Genome Sequences of Three Uropathogenic Klebsiella quasipneumoniae Strains Isolated from Postmenopausal Women with Recurrent Urinary Tract Infection.

Venkitapathi Sundharamani S Sharon Belle M BM Ratna Tahira A TA Arute Amanda P AP Zimmern Philippe E PE De Nisco Nicole J NJ

Microbiology resource announcements 20210318 11

Recurrent urinary tract infection (rUTI) poses a major health issue, especially among postmenopausal women. We report complete genome sequences of three Klebsiella quasipneumoniae strains isolated from the urine of postmenopausal women with rUTI. K. quasipneumoniae is a recently identified Klebsiella species with clinical and virulence characteristics distinct from those of K. pneumoniae. ...[more]

PMID: 33737357

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Project description:IntroductionRecurrent urinary tract infections (RUTIs) caused by uropathogenic Escherichia coli are costly public health problems impacting patients' quality of life.AimIn this work, a comparative genomics analysis of three clinical RUTI strains isolated from bladder biopsy specimens was performed.Materials and methodsOne hundred seventy-two whole genomes of urinary tract E. coli strains were selected from the NCBI database. The search for virulence factors, fitness genes, regions of interest, and genetic elements associated with resistance was manually carried out. The phenotypic characterization of antibiotic resistance, haemolysis, motility, and biofilm formation was performed. Moreover, adherence and invasion assays with human bladder HTB-5 cells, and transmission electron microscopy (TEM) were performed.ResultsThe UTI-1_774U and UTI-3_455U/ST1193 strains were associated with the extraintestinal pathotypes, and the UTI-2_245U/ST295 strain was associated with the intestinal pathotype, according to a phylogenetic analysis of 172 E. coli urinary strains. The three RUTI strains were of clinical, epidemiological, and zoonotic relevance. Several resistance genes were found within the plasmids of these strains, and a multidrug resistance phenotype was revealed. Other virulence genes associated with CFT073 were not identified in the three RUTI strains (genes for type 1 and P fimbriae, haemolysin hlyA, and sat toxin). Quantitative adherence analysis showed that UTI-1_774U was significantly (p < 0.0001) more adherent to human bladder HTB-5 cells. Quantitative invasion analysis showed that UTI-2_245U was significantly more invasive than the control strains. No haemolysis or biofilm activity was detected in the three RUTI strains. The TEM micrographs showed the presence of short and thin fimbriae only in the UTI-2_245U strain.ConclusionThe high variability and genetic diversity of the RUTI strains indicate that are a mosaic of virulence, resistance, and fitness genes that could promote recurrence in susceptible patients.

Project description:BackgroundUropathogenic Escherichia coli (UPEC) is the predominant cause of urinary tract infections (UTIs), and the recurrence of these infections poses significant treatment challenges.ObjectiveThis study aimed to compare the phylogroups, biofilm formation, virulence factors, and antibiotic resistance of UPEC strains in patients with recurrent versus non-recurrent UTIs in Hamadan City, Western Iran.Materials and methodsA total of 110 E. coli isolates were collected from urine cultures across three major hospitals and laboratories. The isolates were confirmed through biochemical tests, and their antibiotic resistance profiles were evaluated using the disk diffusion method. Biofilm production was assessed using the microtiter plate method, while virulence genes and phylogroup determination were analyzed via PCR. Real-time PCR was employed to compare the expression levels of the pap and fimH virulence genes.ResultsThe results indicated that 73% of isolates were from non-recurrent UTI patients, with a higher incidence in females and children under 10 years. A significant difference was detected in the underlying diseases and the expression of the pap between the recurrent and non-recurrent groups. Antibiotic resistance was notably significant, particularly against Ampicillin-sulbactam, Trimethoprim-Sulfamethoxazole, Nalidixic acid, and Ciprofloxacin, with 77% of strains classified as multi-drug resistant (MDR). Despite differences in the rates of ESBL production between recurrent (53%) and non-recurrent (42.5%) strains, no significant differences were observed in antibiotic resistance, biofilm formation, virulence factors, or phylogroups between the two groups. Phylogenetic analysis revealed a predominance of phylogroups B2 and D, with high genetic diversity among the isolates.ConclusionThe study highlights the traits of UPEC strains in recurrent and non-recurrent UTIs, showing high antibiotic resistance and genetic diversity among isolates. The study found notable differences in underlying diseases and the expression of the pap gene between recurrent and non-recurrent groups, suggesting that these factors may play a crucial role in the recurrence of infections. Further investigation into these differences could enhance our understanding and management of recurrent UTIs.

Dataset Information

Complete Genome Sequences of Three Uropathogenic Klebsiella quasipneumoniae Strains Isolated from Postmenopausal Women with Recurrent Urinary Tract Infection.

Publications

Complete Genome Sequences of Three Uropathogenic Klebsiella quasipneumoniae Strains Isolated from Postmenopausal Women with Recurrent Urinary Tract Infection.

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