Assessment of estrogenic activity in PM₁₀ air samples with the ERE-CALUX bioassay: Method optimization and implementation at an urban location in Flanders (Belgium).

ABSTRACT: Endocrine disrupting chemicals represent a broad class of compounds, are widespread in the environment and can pose severe health effects. The objective of this study was to investigate the overall estrogen activating potential of PM10 air samples at an urban location with high traffic incidence in Flanders, using a human in vitro cell bioassay. PM10 samples (n = 36) were collected on glass fiber filters every six days between April 2013 and January 2014 using a high-volume sampler. Extraction was executed with a hexane/acetone mixture before analysis using a recombinant estrogen-responsive human ovarian carcinoma (BG1Luc4E2) cell line. In addition, several samples and procedural blanks were extracted with ultra-pure ethanol or acetonitrile to compare extraction efficiencies. Results were

SUBMITTER: Croes K 

PROVIDER: S-EPMC7976781 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature