A platform for post-translational spatiotemporal control of cellular proteins.

ABSTRACT: Mammalian cells process information through coordinated spatiotemporal regulation of proteins. Engineering cellular networks thus relies on efficient tools for regulating protein levels in specific subcellular compartments. To address the need to manipulate the extent and dynamics of protein localization, we developed a platform technology for the target-specific control of protein destination. This platform is based on bifunctional molecules comprising a target-specific nanobody and universal sequences determining target subcellular localization or degradation rate. We demonstrate that nanobody-mediated localization depends on the expression level of the target and the nanobody, and the extent of target subcellular localization can be regulated by combining multiple target-specific nanobo

SUBMITTER: Jayanthi B 

PROVIDER: S-EPMC7976946 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature