Neutrophil extracellular trap clearance by synovial macrophages in gout.

ABSTRACT:

Background

Monosodium urate (MSU) crystals, i.e., the central etiological factors in gouty arthritis, induce the formation of neutrophil extracellular traps (NETs). We investigated whether synovial macrophages could clear NETs as a self-resolution mechanism in acute gouty arthritis.

Methods

Synovial fluid mononuclear cells (SFMCs) were incubated with NETs induced by MSU crystals. NET engulfment was determined based on neutrophil elastase (NE), myeloperoxidase (MPO), and SYTOX Green signals within synovial fluid CD14⁺ cells. In addition, the correlations between CD14⁺ cells, MPO-dsDNA complexes, and expression of pro- and anti-inflammatory cytokines were analyzed in the synovial fluid CD14⁺ macrophages of patients with gouty arthritis.

Resu

SUBMITTER: Jeong JH 

PROVIDER: S-EPMC7977263 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature