The subcellular redistribution of NLRC5 promotes angiogenesis via interacting with STAT3 in endothelial cells.

ABSTRACT: Angiogenesis is a critical step in repair of tissue injury. The pattern recognition receptors (PRRs) recognize pathogen and damage associated molecular patterns (DAMPs) during injury and achieve host defense directly. However, the role of NLR family CARD domain containing 5 (NLRC5), an important member of PPRs, beyond host defense in angiogenesis during tissue repair remains unknown. Methods:In vitro, western blot and real-time PCR (RT-PCR) were used to detect the expression of NLRC5 in endothelial cells (ECs). Immunofluorescence microscopy was used to reveal the subcellular location of NLRC5 in ECs. Cell proliferation, wound healing, tube formation assays of ECs were performed to study the role of NLRC5 in angiogenesis. By using Tie2Cre-NLRC5^flox/flox mice and bon

SUBMITTER: Xu X 

PROVIDER: S-EPMC7977449 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature