Single-cell transcriptome analysis of the heterogeneous effects of differential expression of tumor PD-L1 on responding TCR-T cells.

ABSTRACT: Rationale: TCR-T cell therapy plays a critical role in the treatment of malignant cancers. However, it is unclear how TCR-T cells are affected by PD-L1 molecule in the tumor environment. We performed an in-depth evaluation on how differential expressions of tumor PD-L1 can affect the functionality of T cells. Methods: We used MART-1-specific TCR-T cells (TCR-T_MART-1), stimulated with MART-1_27-35 peptide-loaded MEL-526 tumor cells, expressing different proportions of PD-L1, to perform cellular assays and high-throughput single-cell RNA sequencing. Results: Different clusters of activated or cytotoxic TCR-T_MART-1 responded divergently when stimulated with tumor cells expressing different percentages of PD-L1 expression. Compared to contro

SUBMITTER: Ding R 

PROVIDER: S-EPMC7978322 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature