Validation of a commercially available indirect assay for SARS-CoV-2 neutralising antibodies using a pseudotyped virus assay.

ABSTRACT:

Objectives

To assess whether a commercially available CE-IVD, ELISA-based surrogate neutralisation assay (cPass, Genscript) provides a genuine measure of SARS-CoV-2 neutralisation by human sera, and further to establish whether measuring responses against the RBD of S was a diagnostically useful proxy for responses against the whole S protein.

Methods

Serum samples from 30 patients were assayed for anti-NP responses, for 'neutralisation' by the surrogate neutralisation assay and for neutralisation by SARS-CoV-2 S pseudotyped virus assays utilising two target cell lines. Correlation between assays was measured using linear regression.

Results

The responses observed within the surrogate neutralisation assay demonstrated an extremely strong, highly significant positive c

SUBMITTER: Murray MJ 

PROVIDER: S-EPMC7979278 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature