Ribonuclease 7-driven activation of ROS1 is a potential therapeutic target in hepatocellular carcinoma.

ABSTRACT:

Background & aims

There are currently limited therapeutic options for hepatocellular carcinoma (HCC), particularly when it is diagnosed at advanced stages. Herein, we examined the pathophysiological role of ROS1 and assessed the utility of ROS1-targeted therapy for the treatment of HCC.

Methods

Recombinant ribonucleases (RNases) were purified, and the ligand-receptor relationship between RNase7 and ROS1 was validated in HCC cell lines by Duolink, immunofluorescence, and immunoprecipitation assays. Potential interacting residues between ROS1 and RNase7 were predicted using a protein-protein docking approach. The oncogenic function of RNase7 was analyzed by cell proliferation, migration and invasion assays, and a xenograft mouse model. The efficacy of anti-ROS1 inhibitor treat

SUBMITTER: Liu C 

PROVIDER: S-EPMC7979498 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature