Canonical and early lineage-specific stem cell types identified in planarian SirNeoblasts.

ABSTRACT:

Background

The pluripotent stem cells in planarians, a model for tissue and cellular regeneration, remain further identification. We recently developed a method to enrich piwi-1+ cells in Schmidtea mediterranea, by staining cells with SiR-DNA and Cell Tracker Green, named SirNeoblasts that permits their propagation and subsequent functional study in vivo. Since traditional enrichment for planarian neoblasts by Hoechst 33342 staining generates X1 cells, blocking the cell cycle and inducing cytotoxicity, this method by SiR-DNA and Cell Tracker Green represents a complementary technological advance for functional investigation of cell fate and regeneration. However, the similarities in heterogeneity of cell subtypes between SirNeoblasts and X1 remain unknown.

Results

In this wo

SUBMITTER: Niu K 

PROVIDER: S-EPMC7979843 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature