A single donor is sufficient to produce a highly functional in vitro antibody library.

ABSTRACT: Antibody complementarity determining region diversity has been considered to be the most important metric for the production of a functional antibody library. Generally, the greater the antibody library diversity, the greater the probability of selecting a diverse array of high affinity leads. According to this paradigm, the primary means of elevating library diversity has been by increasing the number of donors. In the present study we explored the possibility of creating an in vitro antibody library from a single healthy individual, showing that the number of lymphocytes, rather than the number of donors, is the key criterion in the production of a diverse and functional antibody library. We describe the construction of a high-quality phage display library comprising 5 × 10⁹ h

SUBMITTER: Erasmus MF 

PROVIDER: S-EPMC7979914 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature