R-loop resolution promotes co-transcriptional chromatin silencing.

ABSTRACT: RNA-mediated chromatin silencing is central to genome regulation in many organisms. However, how nascent non-coding transcripts regulate chromatin is poorly understood. Here, through analysis of Arabidopsis FLC, we show that resolution of a nascent-transcript-induced R-loop promotes chromatin silencing. Stabilization of an antisense-induced R-loop at the 3' end of FLC enables an RNA binding protein FCA, with its direct partner FY/WDR33 and other 3'-end processing factors, to polyadenylate the nascent antisense transcript. This clears the R-loop and recruits the chromatin modifiers demethylating H3K4me1. FCA immunoprecipitates with components of the m⁶A writer complex, and m⁶A modification affects dynamics of FCA nuclear condensates, and promotes FLC chromatin silencin

SUBMITTER: Xu C 

PROVIDER: S-EPMC7979926 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature