TagBiFC technique allows long-term single-molecule tracking of protein-protein interactions in living cells.

ABSTRACT: Protein-protein interactions (PPIs) are critical for cellular activity regulation. Visualization of PPIs using bimolecular fluorescence complementation (BiFC) techniques helps to understand how PPIs implement their functions. However, current BiFC is based on fluorescent proteins and the brightness and photostability are suboptimal for single molecule tracking experiments, resulting in either low spatiotemporal resolution or incapability of tracking for extended time course. Here, we developed the TagBiFC technique based on split HaloTag, a self-labeling tag that could conjugate an organic dye molecule and thus offered better brightness and photostability than fluorescent proteins for PPI visualization inside living cells. Through screening and optimization, we demonstrated that the recons

SUBMITTER: Shao S 

PROVIDER: S-EPMC7979928 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature