Modulating Ocular Surface Pain Through Neurokinin-1 Receptor Blockade.

ABSTRACT:

Purpose

The purpose of this study was to test the role of substance P (SP) and its receptor neurokinin 1 (NK1R) on ocular surface pain.

Methods

Eight-week-old C57BL6/N (wild type [WT]) and B6.Cg-Tac1tm1Bbm/J (TAC1-KO) male mice were used. 5 M NaCl was topically applied on the cornea, followed by topical fosaprepitant 2, 10, and 50 mg/mL; 4 mg/mL oxybuprocaine chloride, or 0.1% diclofenac. Th eye wiping test was used to quantify ocular surface pain. SP content was quantified in the tear fluid and trigeminal ganglia (TG), and TAC1 mRNA was assessed in the cornea. Corneas were immunostained for β3-tubulin and NK1R, or CD45, to quantify leukocyte infiltration.

Results

TAC1-KO mice displayed a significant reduction of ocular pain (P < 0.001). Similarly, a single dose of 10

SUBMITTER: Lasagni Vitar RM 

PROVIDER: S-EPMC7980039 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature