Donor-dependent aging of young and old red blood cell subpopulations: Metabolic and functional heterogeneity.

ABSTRACT:

Background

Characteristics of red blood cells (RBCs) are influenced by donor variability. This study assessed quality and metabolomic variables of RBC subpopulations of varied biologic age in red blood cell concentrates (RCCs) from male and female donors to evaluate their contribution to the storage lesion.

Study design and methods

Red blood cell concentrates from healthy male (n = 6) and female (n = 4) donors were Percoll separated into less dense ("young", Y-RCCs) and dense ("old", O-RCCs) subpopulations, which were assessed weekly for 28 days for changes in hemolysis, mean cell volume (MCV), hemoglobin concentration (MCHC), hemoglobin autofluorescence (HGB), morphology index (MI), oxygen affinity (p50), rigidity, intracellular reactive oxygen species (ROS), calcium ([Ca

SUBMITTER: Mykhailova O 

PROVIDER: S-EPMC7980132 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature